Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Liver injury induced by paracetamol and challenges associated with intentional and unintentional use Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Researchers are studying a possible link between acetaminophen (Tylenol) use during pregnancy and higher autism or ADHD risk. It may lower glutathione, an important antioxidant for brain development.