View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Frontiers Liquid Chromatography Tandem Mass Spectrometry Analysis of Acetaminophen Covalent Binding to Glutathione S Transferases File:Acetaminophen metabolism.jpg Wikimedia Commons Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect